Abstract
Lysozyme was covalently immobilized onto self-assembled monolayer (SAM) 3-mercaptopropanoic acid, linked to hydrothermally synthesized ZnO nanowires. Immobilization was confirmed by atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy and fluorescence microscopy. Adherence of antibodies to immobilized lysozyme ZnO nanowire constructs showed a strong indication of a piezoelectric signal, observed as an increase in voltage due to the presence of a Schottky barrier. The piezoelectric energy generated increased with an increase in antibody levels. The biosensor was studied by AFM, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The response of the piezoelectric nanoforce biosensor is in a linear relationship with antibody concentration ranges from 50ng/ml to 1μg/ml, with a limit of detection (LOD) of 102.76ng antibodies/ml.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
