Abstract
A nanofluidic biosensor based on nanoreplica molding photonic crystal (PC) was proposed. UV epoxy PC was fabricated by nanoreplica molding on a master PC wafer. The nanochannels were sealed between the gratings on the PC surface and a taped layer. The resonance wavelength of PC-based nanofluidic biosensor was used for testing the sealing effect. According to the peak wavelength value of the sensor, an initial label-free experiment was realized with R6g as the analyte. When the PC-based biosensor was illuminated by a monochromatic light source with a specific angle, the resonance wavelength of the sensor will match with the light source and amplified the electromagnetic field. The amplified electromagnetic field was used to enhance the fluorescence excitation result. The enhancement effect was used for enhancing fluorescence excitation and emission when matched with the resonance condition. Alexa Fluor 635 was used as the target dye excited by 637-nm laser source on a configured photonic crystal enhanced fluorescence (PCEF) setup, and an initial PCEF enhancement factor was obtained.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-016-1644-x) contains supplementary material, which is available to authorized users.
Highlights
Biosensors, which are used for collecting information from interaction between biomolecules and related sensor environment, have been researched extensively in healthcare, biomedical, and life science area [1,2,3,4,5,6,7,8]
The transmission spectrum setup used for testing is shown in Fig. 5; a white light source is collimated by a collimation lens and Transverse magnetic (TM) polarized by a polarizer
Flow ability of the analyte was analyzed based of the peak wavelength shift of the photonic crystal (PC)-based biosensor
Summary
Biosensors, which are used for collecting information from interaction between biomolecules and related sensor environment, have been researched extensively in healthcare, biomedical, and life science area [1,2,3,4,5,6,7,8]. Optical biosensors with intrinsically characteristics of labelfree, high signal-to-noise ratio, and easy to integrate are even indispensable in low-concentration detection experiments [9,10,11,12,13]. Traditional mass transport optical biosensors are very difficult for the analytes to attach to the sensing surface through convection flow and diffusion [14, 15]. Nanochannel has a very high surfaceto-volume ratio, which makes it much easier for analyte molecules to bump into the internal surfaces, where it can be captured by recognition molecules such as antibody [16,17,18,19,20]. For low-concentration analytes, the time required for analytes to touch the sensing surface can take many hours with normal-size channels by diffusion, which is an important limitation to the performance of surface biosensors.
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