Abstract
Hydrogen peroxide (H2O2) plays important signaling roles in normal physiology and disease. However, analyzing the actions of H2O2 is often impeded by the difficulty in detecting this molecule. Herein, we report a novel nanocomposite-based electrochemical sensor for non-enzymatic detection of H2O2. Graphene oxide (GO) was selected as the dopant for the synthesis of polyaniline (PANI), leading to the successful fabrication of a water-soluble and stable GO-PANI composite. GO-PANI was subsequently subject to cyclic voltammetry to generate reduced GO-PANI (rGO-PANI), enhancing the conductivity of the material. Platinum nanoparticles (PtNPs) were then electrodeposited on the surface of the rGO-PANI-modified glassy carbon electrode (GCE) to form an electrochemical H2O2 sensor. Compared to previously reported sensors, the rGO-PANI-PtNP/GCE exhibited an expanded linear range, higher sensitivity, and lower detection limit in the quantification of H2O2. In addition, the sensor displayed outstanding reproducibility and selectivity in real-sample examination. Our study suggests that the rGO-PANI-PtNP/GCE may have broad utility in H2O2 detection under physiological and pathological conditions.
Highlights
Hydrogen peroxide (H2O2) is a signaling molecule critically involved in various physiological and pathological processes, such as cell migration, cell proliferation, immune response, and circadian rhythm [1, 2]
Our study suggests that the rGO-PANI-Platinum nanoparticles (PtNPs)/glassy carbon electrode (GCE) may have broad utility in H2O2 detection under physiological and pathological conditions
The rGO-PANI-PtNP/GCE H2O2 sensor was prepared by electrodepositing PtNPs on the surface of the rGO-PANI-coated GCE (Scheme 1)
Summary
Hydrogen peroxide (H2O2) is a signaling molecule critically involved in various physiological and pathological processes, such as cell migration, cell proliferation, immune response, and circadian rhythm [1, 2]. The difficulty of detecting H2O2 has been an obstacle in investigating its involvement in health and disease. Over the past few decades, a myriad of methods for analytical quantification of H2O2 have been developed, based on mass spectrometry [3], fluorescence [4], and chemiluminescence [5]. Electrochemical sensors have been developed for the determination of H2O2. Most of the electrochemical sensors are based on enzymes and fraught with issues that include low reproducibility and high instability, because enzymes require specific environ mental conditions to maintain their activity [6]. The preparation of non-enzymatic sensors for detecting H2O2 is believed to have broader applications
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