Abstract
The herbicide residue from intensive agricultural activity provokes environmental disturbances and human health injuries. Among the enzymatic disruptor herbicides, mesotrione is able to inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD), which plays a key role in the carotenoid synthesis. Therefore, enzyme-based sensors are innovative options for monitoring herbicides used in agriculture. Compared to the standard sensors, biosensors have assorted advantages, such as practicality, quick response, low cost, and high sensitivity. A nanobiosensor was developed herein based on HPPD for mesotrione detection. Theoretically, the molecular docking and molecular dynamics simulation estimated the interacting regions of HPPD with mesotrione. Experimentally, the atomic force microscope tip functionalization with HPPD immobilized in self-assembled monolayers was confirmed by fluorescence microscopy and atomic force spectroscopy. The cross-linker N-(3-dimethylaminopropyl)- $\mathrm{N}^\prime $ -ethylcarbodiimide hydrochloride was responsible for properly preserving the enzyme on the tip. The nanobiosensor proposed here was successfully able to detect mesotrione molecules. Such effectiveness in the development of nanobiosensors promises reliable, precise, and low-cost techniques, which apply to a broad range of issues, from ecology to medicine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.