Abstract
A mutation in negative regulator of basal resistance WRKY17 of Arabidopsis increases susceptibility to Agrobacterium-mediated transient genetic transformation
Highlights
WRKY protein family is composed of at least 74 members in Arabidopsis thaliana[1]; they act as transcriptional regulators and participate mainly in the control of gene expression involved in the plant stress response, and, in the induction of gene expression by pathogen-derived elicitors
We investigated the effect of the WRKY17 mutation on the expression of the VIP1 gene
The wrky[17] mutant is hypersusceptible to Agrobacteriummediated genetic transformation Once we had identified plant tissue showing a clear effect of WRKY17 on VIP1 expression, we investigated whether this effect altered susceptibility to Agrobacterium infection
Summary
WRKY protein family is composed of at least 74 members in Arabidopsis thaliana[1]; they act as transcriptional regulators and participate mainly in the control of gene expression involved in the plant stress response, and, in the induction of gene expression by pathogen-derived elicitors. Arabidopsis WRKY17, together with another family member WRKY11, is a negative regulator of the basal defense response[2]. The WRKY17 and WRKY11 genes are usually induced during the defense response, and Arabidopsis loss-of-function mutants wrky[17] and wrky[11] display higher expression of numerous stress- or defense-related genes and show increased resistance to infection by Pseudomonas, but not by other pathogens. The VIP1related defense responses are activated during Agrobacterium-host plant interactions, and Agrobacterium has evolved to subvert them to facilitate the infection process[4,6]
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