Abstract
Abstract Chick embryo fibroblasts infected with a temperature-sensitive mutant of Rous sarcoma virus (chicken embryo fibroblasts (RSV-Ta)) have normal morphology at 40.5° but after a transfer to 36° show the morphological characteristics of transformed cells. This transformation is prevented by N6,2'-O-dibutyryl adenosine 3',5'-monophosphate plus theophylline. Chicken embryo fibroblasts (RSV-Ta) have the same high endogenous levels of adenosine 3',5'-monophosphate (cyclic AMP) at 40.5° as uninfected fibroblasts; however, when shifted to 36° the cyclic AMP levels fall to a value similar to that of the fibroblasts infected with wild type RSV. This suggests that an early event in transformation leads to a decreased level of cyclic AMP, and the decreased level of cyclic AMP results in some of the unique properties of transformed cells.
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