A Multiyear Survey and Identification of Pepper- and Tomato-Infecting Viruses in Yunnan Province, China.

Guanlin Tan,Fan Li,Xiaojiao Chen,Pingxiu Lan,Long Xiao,Wenpeng Zhou,Yueyue Li,Yong Liu,Ruhui Li

doi:10.3389/fmicb.2021.623875

Abstract

During pepper and tomato production seasons in 2013–2017, large-scale virus disease surveys were conducted in different regions of Yunnan Province, China. A total of 1,267 pepper and tomato samples with various virus-like symptoms were collected and analyzed for virus infections through dot enzyme-linked immunosorbent assay (dot-ELISA), polymerase chain reaction (PCR), and reverse-transcription (RT)-PCR. The detection results showed that 19 different viruses were present in about 50.9% of the assayed samples, and among these viruses, seven viruses were found in both pepper and tomato samples. Mixed infections with two to three of the 15 identified mixed infection types were found in the pepper samples and 10 identified mixed infection types were found in the tomato samples. Among the infected samples, Tomato spotted wilt orthotospovirus (TSWV) was the most common virus, with a detection rate of about 20.0% followed by Pepper vein yellows virus (PeVYV, 13.0%). This survey revealed for the first time that pepper is a natural host of Tobacco vein distorting virus (TVDV) worldwide and tomato is a natural host of Potato leafroll virus (PLRV) in China. PeVYV, Tobacco mild green mosaic virus (TMGMV) and Wild tomato mosaic virus (WTMV) were first time found in pepper and Tomato mottle mosaic virus (ToMMV) and Chilli veinal mottle virus (ChiVMV) were first time found in tomato in Yunnan Province. Finally, the virus incidences were higher in Kunming, Yuxi, Chuxiong, and Honghe region than other regions.

Highlights

Pepper (Capsicum annuum) and tomato (Solanum lycopersicum) are two economically important solanaceous vegetable crops in China and many other countries
A total number of 821 pepper samples and 446 tomato samples were collected during the surveys
Our result showed that Tomato spotted wilt orthotospovirus (TSWV), Pepper vein yellows virus (PeVYV), Cucumber mosaic virus (CMV), and Pepper mild mottle virus (PMMoV) occurred every year in the pepper and tomato fields in this province from 2013 to 2016 (Figure 2C)

Summary

Introduction

Pepper (Capsicum annuum) and tomato (Solanum lycopersicum) are two economically important solanaceous vegetable crops in China and many other countries. Pepper and Tomato Virus Diseases yield losses. Pepper and tomato are the two major solanaceous vegetable crops in Yunnan Province. Some pepper and tomato fields in this province had total yield losses in the recent years, resulting in a quick increase of pesticide applications, insect tolerance to pesticides, and environment pollutions (Chen and Liu, 2013; Bu et al, 2014)

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Discussion

Conclusion