Abstract
We report the development of a multiwell biosensor for detecting changes in the electrochemical open circuit potential (OCP) generated by viable human cells in vitro. The instrument features eight culture wells; each containing three gold sensors around a common silver/silver chloride reference electrode, prepared using screen-printed conductive inks. The potential applications of the device were demonstrated by monitoring rheumatoid synovial fibroblasts (RSF) and HepG2 hepatocarcinoma cells in response to chemical and biological treatments. This technology could provide an alternative to conventional end-point assays used in the fields of chemotherapy, toxicology and drug discovery.
Highlights
We have previously reported the principles of an electrochemical biosensor, ‘Oncoprobe’, for passively recording the Electrochemical open circuit potential (OCP) of confluent cell monolayers on gold sensors in vitro and its application in anticancer drug testing [1,2,3]
We report on developments on the original assay design, whereby the solid gold electrodes and calomel reference electrodes previously employed have been replaced with a miniaturised disposable ceramic probe with screen-printed gold sensors and silver/silver chloride reference electrodes suitable for an eight-well assay device
We demonstrate the application of the technology using in vitro cell cultures of synovial fibroblasts and the human hepatocellular carcinoma cell line, HepG2
Summary
We have previously reported the principles of an electrochemical biosensor, ‘Oncoprobe’, for passively recording the Electrochemical open circuit potential (OCP) of confluent cell monolayers on gold sensors in vitro and its application in anticancer drug testing [1,2,3]. Changes in cell behaviour, such as those induced by anticancer drugs, inflammatory cytokines or other agents were shown to modify the OCP of viable cells in vitro. Biosensor technologies have been developed to overcome the limitations of these assays and several approaches have been taken to achieve this; including impedance [8], quartz crystal microbalance [9,10], optical [11], ion selective [12] and electrochemical methods [13] of detection. We demonstrate the application of the technology using in vitro cell cultures of synovial fibroblasts and the human hepatocellular carcinoma cell line, HepG2
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
