A Multiprotein Complex That Interacts with RNA Polymerase II Elongator

Yang Li,Yuichiro Takagi,Yiwei Jiang,Masao Tokunaga,Hediye Erdjument-Bromage,Paul Tempst,Roger D Kornberg

doi:10.1074/jbc.c100274200

Abstract

A three-subunit Hap complex that interacts with the RNA polymerase II Elongator was isolated from yeast. Deletions of genes for two Hap subunits, HAP1 and HAP3, confer pGKL killer-insensitive and weak Elongator phenotypes. Preferential interaction of the Hap complex with free rather than RNA polymerase II-associated Elongator suggests a role in the regulation of Elongator activity.

Highlights

From the ‡Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305-5400, ¶Department of Medical Biochemistry and Genetics, Texas A & M University System Health Science Center, College Station, Texas 77843-1114, ʈApplied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Kagoshima 8900065, Japan, and **Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021
A three-subunit Hap complex that interacts with the RNA polymerase II Elongator was isolated from yeast
Transcription by RNA polymerase II in eukaryotic cells is regulated at all levels, including reorganization of the chromatin template and transcription initiation, elongation, and termination [1]

Summary

Introduction

From the ‡Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305-5400, ¶Department of Medical Biochemistry and Genetics, Texas A & M University System Health Science Center, College Station, Texas 77843-1114, ʈApplied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Kagoshima 8900065, Japan, and **Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021. Printed in U.S.A. A Multiprotein Complex That Interacts with RNA Polymerase II Elongator* A three-subunit Hap complex that interacts with the RNA polymerase II Elongator was isolated from yeast. Preferential interaction of the Hap complex with free rather than RNA polymerase II-associated Elongator suggests a role in the regulation of Elongator activity.

Results

Conclusion