Abstract
The use of polyethylene containers eliminates many disadvantages of those made of glass. A container consisting of 35 individual units (baskets) mounted vertically in a polyethylene disk 43½4 inches in diameter and 1½8 inch thick was a very successful substitute for glass baskets when used in Schneider's (1952) shaker-staining method. Each basket was made from a 2-inch length of 56-inch outside diameter and ½-inch inside diameter polyethylene tubing, with its bottom end closed by a fused-in circular piece of 100-mesh nickel screen. The baskets were inserted through tight holes bored in the disk by a No. 10 cork borer. The multiple-unit assembly was fitted with a handle made of polyethylene rods. Staining was the same as that obtained in glass baskets and polyethylene was found to resist the usual histological reagents at temperatures below 70 °C.
Published Version
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