Abstract
In this paper, a multifunctional biosensing platform for sensitively detecting Hg2+ and Ag+, based on ion-mediated base mismatch, fluorescent labeling, and strand displacement, is introduced. The sensor can also be used as an OR logic gate, the multifunctional design of sensors is realized. Firstly, orthogonal experiments with three factors and three levels were carried out on the designed sensor, and preliminary optimization of conditions was performed for subsequent experiments. Next, the designed sensor was tested the specificity and target selectivity under the optimized conditions, and the application to actual environmental samples further verified the feasibility. Generally, this is a convenient, fast, stable, and low-cost method that provides a variety of ideas and an experimental basis for subsequent research.
Highlights
With the further development of science and the deepening of research in various disciplines, we have found that some problems cannot be solved perfectly though a single subject, so the concept and research methods of interdisciplinary subjects have emerged
Constructing simple molecular logic gates [7] and interconnecting the relationships between these logic gates and a more complex logic loop is the basis for building biocomputers
The method satisfies the purpose of constructing various logic gates [13] and overcomes the defects in current detection methods and realize the functional connection between molecular logic gates and biosensor functions
Summary
With the further development of science and the deepening of research in various disciplines, we have found that some problems cannot be solved perfectly though a single subject, so the concept and research methods of interdisciplinary subjects have emerged. The conventional methods for detecting heavy metal ions include cold vapor atomic fluorescence spectrometry [25], cold atomic absorption spectrometry [26], inductively coupled plasma mass spectrometry [27], and X-ray spectrometry [28]. The development of convenient, fast, and cost-effective methods for detecting heavy metal ions in the environment has become one of the hot issues in current research. Ag+ have been proposed, such as the G-quadruplexes detection method [32,33], the fluorescence detection method [34,35], the electrochemical detection method [11,36,37,38,39], the DNA-modified gold nanoparticles detection method [11,16,21,40], the colorimetry detection method [41,42] and the probe microscope detection method [43] These methods have the advantages of low cost, high efficiency, time efficiency, simple operation, high sensitivity, and specificity, and they completely overcome the disadvantages of traditional detection methods [44]. In the orthogonal experiment, condition optimization and performance testing were carried out to verify the feasibility and practical significance of the design
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