Abstract
We have developed a multi-channel electrochemical immunosensing system for the detection of α-fetoprotein(AFP).The sensing system consisted of carbon screen-printed electrode(SPCE) arrays and poly HRP-based signal amplification probes.AFP antigens could bind to both capture antibodies immobilized on electrode arrays and detection antibodies(anti-AFP Ig G developed in rabbit). The anti-rabbit Ig G conjugated to poly-horseradish peroxidase were used as a signaling probe to bind to the immuno-complex. The current signals were harvested by a home-made multi-channel electrochemical detector. AFP could be detected in the concentration ranges of 64 pg·m L-1~ 250 ng·m L-1. The detection limit was as low as 56 pg·m L-1. The proposed immunosensing system provided a high sensitive, specific, simple and practical method for AFP detection.
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