Abstract

We describe a mechanical configuration that allows any point on a flat sample to be moved to any angle of tilt and rotation (within the limits of which the stage is capable) while maintaining the area of interest at a constant focus and within the field of view of a fixed optical system. We describe eight axes of movement required to obtain the desired stability of focus and view area. We demonstrate the proposed concept by assembling an instrument using mostly existing parts. This optical-microscope-based instrument allows sample tilts to 60° and unlimited rotation while maintaining the point of interest within better than 17μm in x, y, and z of the center of the field of view of a 100× optical system.

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