Abstract

An ATP bioluminesce assay (ATP Water Test), measuring organic bioburden including microbes, was used to monitor the cleanliness of flexible GI endoscopes after the manual cleaning step of the decontamination and disinfection process, in seven US hospitals.

Highlights

  • An ATP bioluminesce assay (ATP Water Test), measuring organic bioburden including microbes, was used to monitor the cleanliness of flexible GI endoscopes after the manual cleaning step of the decontamination and disinfection process, in seven US hospitals

  • The level of ATP contamination post manual cleaning was found to be statistically different by scope type (p

  • Using the 200 relative light units (RLUs) value proposed in the literature1,2 as a pass-fail cleanliness criterion after manual cleaning, the observed failure rates were found to be highest for duodenoscopes (31%, 15/48) and gastroscopes (22%, 38/168) and lowest for colonoscopes (3%, 6/182)

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Summary

Introduction

An ATP bioluminesce assay (ATP Water Test), measuring organic bioburden including microbes, was used to monitor the cleanliness of flexible GI endoscopes after the manual cleaning step of the decontamination and disinfection process, in seven US hospitals. Objectives To characterize the effectiveness of the manual cleaning step during reprocessing of flexible endoscopes, from site to site as well as by type of endoscope. Methods Duodenoscopes, gastroscopes, and colonoscopes were tested using an ATP Water Test.

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