Abstract
Cardiac biomarkers (CBs) are substances that appear in the blood when the heart is damaged or stressed. Measurements of the level of CBs can be used in course of diagnostics or monitoring the state of the health of group risk persons. A multi-region bio-analytical system (MRBAS) based on magnetoimpedance (MI) changes was proposed for ultrasensitive simultaneous detection of CBs myoglobin (Mb) and C-reactive protein (CRP). The microfluidic device was designed and developed using standard microfabrication techniques for their usage in different regions, which were pre-modified with specific antibody for specified detection. Mb and CRP antigens labels attached to commercial Dynabeads with selected concentrations were trapped in different detection regions. The MI response of the triple sensitive element was carefully evaluated in initial state and in the presence of biomarkers. The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of CBs. Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. The linear detection range contained low concentration detection area and high concentration detection area, which were 1 pg/mL–10 ng/mL, 10–100 ng/mL for CRP, and 0.1 pg/mL–1 ng/mL, 1 n/mL–80 ng/mL for Mb. The measurement technique presented here provides a new methodology for multi-target biomolecules rapid testing.
Highlights
Measurements of the level of cardiac biomarkers (CBs) is important when the heart is damaged or stressed, especially in the case of cardiovascular disease (CVD) risk [1,2]
In our previous work [25], we found that Dynabeads on the surface of the element were nearly magnetically saturated
The results indicated the selective binding of C-reactive protein (CRP) in detection region 1 to the CRP antibody and CRP antibody and Mb in detection region 2 to the Mb antibody
Summary
Measurements of the level of cardiac biomarkers (CBs) is important when the heart is damaged or stressed, especially in the case of cardiovascular disease (CVD) risk [1,2]. Among various kinds of cardiac biomarkers, C-reactive protein (CRP) is a most important biomarker for CVD risk. 90 pg/mL to 250 ng/mL within 90 min after acute myocardial infarction (AMI), playing a major role in urgent diagnosis of CVD [4,5,6]. Sensors 2018, 18, 1765 biosensors to detect the levels of two biomarkers [7,8,9,10,11]. The surface of the biosensors suffer from perturbations on the sensor surface that are influenced by different pH, ionic strength and co-existing molecules in biological fluids
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