Abstract
Increasing evidence suggests that periodontitis, characterized by oral dysbiosis, is a critical player in the progression of multiple systemic diseases in humans. However, there is still a lack of a proper mouse model of periodontitis with the colonization of human periodontitis-associated bacteria. We here established a new mouse periodontitis model by combining ligation of the second molars with application of subgingival plaques from periodontitis patients. Using 16S rRNA gene sequencing and Taxonomic classification, we found that human periodontitis-associated bacteria efficiently colonized in the mouse model and were enriched in both ligature silk and mouse saliva. Furthermore, the well-recognized periodontal pathogens including Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia, and Tannerella forsythia were enriched in the new model, but not in ligature-induced periodontitis model or Sham mice. The human periodontitis-associated bacteria potently aggravated mouse periodontitis, as demonstrated by more severe bone resorption and higher expression of inflammatory and osteoclastogenesis genes. In summary, the new mouse periodontitis model paves the way for studying human periodontitis-associated bacteria in oral diseases and systemic diseases.
Highlights
Human periodontitis-associated bacteria cause local destruction of periodontal tissue and are tightly linked to the progression of multiple systemic diseases
Human Periodontitis-Causing Bacteria Efficiently Colonize in the Mouse Model
We first compared the numbers of same bacteria in the ligature silk and saliva as in human subgingival plaque in both ligature-induced mouse periodontitis (LIP) and LIP + SP groups
Summary
Human periodontitis-associated bacteria cause local destruction of periodontal tissue and are tightly linked to the progression of multiple systemic diseases. Periodontitis (PD) is a biofilminduced chronic inflammatory disease of the tooth-supporting tissues, which destroys gingiva and alveolar bone, eventually causes teeth loss (Brown LJ and Löe, 1989). The number of new periodontitis cases is about 701/100,000 each year, and periodontitis becomes the 6th most prevalent diseases all over the world (Kassebaum et al, 2014). Some studies suggest that the dysbiosis of oral microecology causes periodontitis, but affects other organs (Hajishengallis, 2015). Accumulating evidence has linked periodontal disease with cardiovascular diseases, metabolic diseases, and cancers (Michaud et al, 2017; Polak and Shapira, 2018; Sanz et al, 2020). Increasing attentions are paid on the human oral dysbiosis during understanding
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
