Abstract
Methods Female C3H/HeJ mice were gavaged at days 0, 1, 2, 7, and 21 with fenugreek in doses ranging from 0.1 mg to 10 mg. Cholera toxin (CT) was used as an adjuvant (10 μg/mouse). The mice were euthanized on day 28, and sera were analysed for fenugreek-specific IgE using the heterologous passive cutaneous anaphylaxis assay. Based on this dose-response experiment, twenty four mice were gavaged with the optimal dose of fenugreek (4.2 mg) and CT at days 0, 1, 2, 7, 21 and 28. Eight mice were gavaged with CT only and 8 mice were not treated. At day 35 all mice received a large dose of fenugreek (25.0 mg) and clinical reactions were observed.
Highlights
Fenugreek (Trigonella foenum-graecum L.) is widely used in Indian-style spiced foods
The mice were euthanized on day 28, and sera were analysed for fenugreek-specific IgE using the heterologous passive cutaneous anaphylaxis assay. Based on this dose-response experiment, twenty four mice were gavaged with the optimal dose of fenugreek (4.2 mg) and Cholera toxin (CT) at days 0, 1, 2, 7, 21 and 28
At day 35 all mice received a large dose of fenugreek (25.0 mg) and clinical reactions were observed
Summary
Fenugreek (Trigonella foenum-graecum L.) is widely used in Indian-style spiced foods. Some cases of allergic reactions to fenugreek have been reported, and a Norwegian study found cross-reactivity of fenugreek in peanut allergic individuals. We wanted to develop a mouse model of fenugreek allergy. Published: 12 August 2011 doi:10.1186/2045-7022-1-S1-P48 Cite this article as: Vinje et al.: A mouse model of fenugreek allergy. Clinical and Translational Allergy 2011 1(Suppl 1):P48
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