A mouse embryonic stem cell culture system with stable and regulatable expression miR-3099: an in vitro approach towards functional genomics study

Abstract

Event Abstract Back to Event A mouse embryonic stem cell culture system with stable and regulatable expression miR-3099: an in vitro approach towards functional genomics study Shahidee Zainal Abidin1, 2, 3*, Jia-Wen Leong1, 2, 4, Norshariza Nordin2, 3, Syahril Abdullah2, 3, Pike-See Cheah1, 2, 4 and King-Hwa Ling1, 2, 3* 1 Universiti Putra Malaysia, NeuroBiology and Genetics Group, Faculty of Medicine and Health Sciences, Malaysia 2 Universiti Putra Malaysia, Genetics and Regenerative Medicine Research Center, Faculty of Medicine and Health Sciences, Malaysia 3 Universiti Putra Malaysia, Medical Genetics Unit, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Malaysia 4 Universiti Putra Malaysia, Department of Human Anatomy, Faculty of Medicine and Health Sciences, Malaysia miR-3099 is a small non-coding RNA that is highly expressed throughout embryogenesis and is localised in cortical plate between E13.5 and E17.5 in developing mouse brain. Majority of the cells in the brain at these time-points are committed to neuronal cell lineage. To understand more on the role of miR-3099 during neurogenesis, this study aims to characterise the role of miR-3099 via in vitro approach with respect to functional genomic study. The bioinformatics study was used to predict the downstream-targeted genes by using four independent prediction software (miRDB, miRanda, TargetScan and DIANA micro-T CDS). The target genes that were predicted by at least three different databases were subjected to DAVID bioinformatics analysis to understand their function in biological processes followed by Allen Brain Atlas expression profile analysis. Based on the analysis, it was suggested that miR-3099 was potentially involved in neural stem cell (NSC) fate determination pathway. This warrants further characterisation by generating mouse embryonic stem (mES) cell with regulatable expression miR-3099 to perform function study at molecular level. Interestingly, miR-3099 seed region was found to be 100% identical with chr21:40558602-40558624 in human genome. This indicates that, by extrapolating the finding from animal model will contribute to a more detailed knowledge of the molecular mechanisms of NSC fate determination toward specific lineages. Acknowledgements The study was supported by Malaysian Ministry of Higher Education's Fundamental Research Grant Scheme awarded to P.-S. Cheah (FRGS/1/2015/SKK08/UPM/02/1) and Malaysian Ministry of Science, Technology and Innovation's Sciencefund grant awarded to K.-H. Ling (02-01-04-SF2336). Keywords: Cerebral Cortex, Genomics, miRNA, in vitro, embryonic stem cell Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: O03: Postgraduate Travel Awardees Oral Session 3 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Zainal Abidin S, Leong J, Nordin N, Abdullah S, Cheah P and Ling K (2016). A mouse embryonic stem cell culture system with stable and regulatable expression miR-3099: an in vitro approach towards functional genomics study. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00091 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Mr. Shahidee Zainal Abidin, Universiti Putra Malaysia, NeuroBiology and Genetics Group, Faculty of Medicine and Health Sciences, Serdang, Selangor, Malaysia, shah.68000@gmail.com Dr. King-Hwa Ling, Universiti Putra Malaysia, NeuroBiology and Genetics Group, Faculty of Medicine and Health Sciences, Serdang, Selangor, Malaysia, lkh@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Shahidee Zainal Abidin Jia-Wen Leong Norshariza Nordin Syahril Abdullah Pike-See Cheah King-Hwa Ling Google Shahidee Zainal Abidin Jia-Wen Leong Norshariza Nordin Syahril Abdullah Pike-See Cheah King-Hwa Ling Google Scholar Shahidee Zainal Abidin Jia-Wen Leong Norshariza Nordin Syahril Abdullah Pike-See Cheah King-Hwa Ling PubMed Shahidee Zainal Abidin Jia-Wen Leong Norshariza Nordin Syahril Abdullah Pike-See Cheah King-Hwa Ling Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.