Abstract
SummaryThe distal radial growth plates of six pairs of spider‐affected and non‐affected lambs were compared after light and electron microscopic evaluation. Light microscopy revealed regions within the spider‐affected physes that lacked the distinctly columnized appearance of the normal ones. However, provisional calculation as revealed by von Kossa staining did not differ between the two groups; nor were there marked ultrastructural differences between control and spider lamb growth plate chondrocytes or matrices. The spider growth plates were also studied after concanavalin A and wheat germ agglutinin lectin histochemistry. Intracellular and extracellular staining patterns did not differ from previously reported normal patterns. Finally, SDS‐PAGE analysis of collagen extracted in the native state from spider‐affected cortical bone revealed that it is comprised of type I collagen that is normal in its primary structure; furthermore, there is no type III collagen present.
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