Abstract
A monoclonal antibody to the trichothecene mycotoxin diacetoxyscirpenol (DAS) was produced by a hybridoma, designated 2E5. It secreted antibody of the IgGl subclass and had a detection limit for DAS of 16 ng/ml with a direct enzyme immunoassay on a double antibody solid phase. The relative cross‐reactivities with 3α‐acetyl‐DAS, diacetylverrucarol, neosolaniol, T‐2 tetraol tetraacetate, fusarenon X, T‐2 toxin, and HT‐2 were 2224·5, 53·7, 13·9, 9·2, 6·4, 1·7, 0·6, and 0·35%, respectively.
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