Abstract
A monoclonal antibody (mAb) binding to protein C (PC) heavy chain but not to activated PC was found to inhibit PC activation by free thrombin, suggesting that epitope involved the activation site. Using a set of overlapping synthetic peptides, we confirmed that this mAb recognizes the sequence encompassing the thrombin cleavage site ( 165QVDPRLI 171). Surprisingly, epitope was only accessible in the absence of calcium, half-maximal inhibition of mAb binding occurring at 100 μM Ca 2+. Thus, our antibody provides direct evidence that conformation and/or accessibility of the activation site differ between the apo and Ca 2+-stabilized conformers of PC.
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