Abstract
In the present study, we report the derivation of a monoclonal antibody against a MDR human ovarian carcinoma cell line, A2780.AD (AD) (Rogan et al., 1984), which recognises a cell surface antigen whose association with drug resistance appears independent of P-glycoprotein
Highlights
Departments of 'Oncology and 2Microbiology & Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada; and 3Ontario Cancer Treatment and Research Foundation, Kingston Regional Cancer Centre, King St
One, designated MAb differ in structure as well as in their mechanism of action. 7.4.1, was selected for further study; the antibody secreted by Development of such multidrug resistance (MDR) is a major this hybridoma was IgGI (Boehringer-Mannheim isotype kit)
While overexpression of P-glycoprotein is clearly re- quantitate the degree of reactivity and to confirm the cell sponsible for MDR in some cell systems, it is unlikely that surface location of the reactive epitope, flow cytometry was
Summary
Departments of 'Oncology and 2Microbiology & Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada; and 3Ontario Cancer Treatment and Research Foundation, Kingston Regional Cancer Centre, King St. A monoclonal antibody detecting cell surface epitope on some drug resistant human tumour cell lines Only a subset of patients with drug-resistant tumours (Gold- Since human tumour samples are frequently preserved by stein et al, 1989), support a multifactorial model of MDR fixation, it was of interest to determine whether formalin,
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