A Monitor Calibrator as a Portable Tool for Determination of Luminescent Compounds

Abstract

A monitor calibrator has been tested as a portable, available, and cost-effective tool for the detection of luminescence. Prospects of this device as an all-sufficient analog of an analytical fluorometer have been outlined. Its ability to determine luminescent compounds was demonstrated by the example of rhodamine 3B and fluorescein. The determination is based on simultaneous impulse irradiation of a fluorescent sample with the incorporated continuous-spectrum light emitter in the visible and near UV ranges and registration of the luminescence. The appropriate cuvettes for the analysis and the device sealing film were chosen. The lowest relative detection limit for the developed approach was 0.03 μmol L-1 for rhodamine 3B and 0.01 μmol L <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> for fluorescein; the lowest absolute detection limits were 0.01 and 0.002 nmol for rhodamine 3B and fluorescein, respectively. Using the proposed approach, it was possible to determine the luminescent compounds in tap water.