Abstract
A novel MIP-based electrochemical sensing platform was developed for the ultrasensitive detection of vacuolating cytotoxin A (VacA) toxin of H. pylori bacteria. VacA virulence factor is majorly involved in H. pylori-associated pathogenesis and plays a vital role in the establishment of bacteria inside host gastric cells. Herein, we have developed a SiO2 nanoparticle decorated imprinting polymer-based sensing platform on a screen printed electrode (SPE) that is attributed as a receptor of VacA protein. The molecularly imprinted polymer has been developed by a polymerization process directly over the screen-printed electrode using template VacA antigen and the performance of the fabricated electrode was studied electrochemically. Under the optimized experimental conditions the fabricated VacA-MIP/SiO2 @SPE sensor exhibits high sensitivity (0.304 mA ng−1 ml−1) and a very low limit of detection (0.01 ng ml−1) in a 0.01–100 ng ml−1 linear range. The influence of various other possible interferents in analytical response has also been observed with the successful determination of VacA antigen.
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