A Molecular and Histological study of Turkey birds infected with Toxoplasmosis

Abstract

Toxoplasma gondii parasite has three contagious stages: the oocytes, which is introduced into the external environment with the feces of the infected cats, which later form the spores. The current study depended on detection of T. gondii parasite used to Polymerase chain reaction, as well as the lack of time and effort required to perform them, the result of the test, 10 samples were selected for dissection and investigation of B1 (399bp) in their organs (liver, eye and intestine) using the normal polymerase chain reaction technique. There were (4) samples (40%) of the birds containing the tissues on the gene B1 (399bp) and recorded the highest incidence of the gene in the samples of the liver, where 30% (three samples) and one sample in the small intestine by 10% B1 in the eye samples, the results of the statistical analysis indicated that there were significant differences in the ratio of TWA D gene B1 in the tissues of the bird members under the level of probability of P⩽0.05, In the study of histopathological changes in turkey birds members, the presence of Lesions lesions associated with infection in both the liver and the small intestine was characterized by the presence of congestion in the blood vessels, as well as the expansion of the jibaniat with the loss of the engineering structure of the liver. In the tissue with a degenerative degeneration.