Abstract
A modification of the standard quantitative precipitin test is described. The new procedure, which utilizes polyethylene glycol (PEG) at both the 37°C and 4°C incubation stages, provides a convenient quantitative precipitin test assay which is more reliable and much faster than the standard assay. Moreover, the modified or PEG assay gives a quantitative measure of antibody concentration even when antisera of low titre are tested. The sensitivity of the capillary-tube test is also enhanced when the supernatant solutions contain PEG.
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