Abstract
A method is described which allows the preparation of multigram quantities of mammalian tRNA. The purification scheme consists of a phenol extraction, a preliminary isopropanol fractionation, a second isopropanol fractionation, DEAE-cellulose chromatography, a phenol/SDS extraction, and a deacylation step. The key feature of this procedure is the preliminary isopropanol fractionation, a step which removes the bulk of the alcohol-precipitable impurities.
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