Abstract
Secondary metabolites in mangroves often interfere with RNA extraction yielding poor concentration and quality, which is unsuitable for downstream applications. As existing protocols yielded low-quality RNA from root tissues of Kandelia candel (L.) Druce and Rhizophora mucronata Lam., an optimized method was developed for improving the quality and yield of RNA. Compared with three other methods, thisoptimizedprotocol gave better RNA yield and purity for both species. The absorbance ratios were ≥1.9 for A260/280 and A260/230, while RNA integrity number values ranged from 7.5 to 9.6. Results show that ourmodified method is efficient in obtaining high-quality RNA from mangrove rootsand is suitable for downstream experiments such as cDNA synthesis, real-timequantitativePCRand next-generation sequencing.
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