Abstract
A modified anaerobic medium and cultivation conditions for Chloroflexaceae are described which assured almost complete anaerobiosis for growth and maintenance. All tested strains of Chloroflexus aurantiacus and Chloroflexus aggregans were successfully grown within relatively short times with full pigmentation, indicating that the new medium and reduced cultivation conditions were most suited for photoautotrophic growth. The anaerobic conditions and the presence of sulfide also favored prolonged viability during maintenance as liquid cultures.
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