A Modified Ink Staining Procedure for Cryptococcus Detection of the Cerebrospinal Fluid by Microscope.

Abstract

The pathogen detection rate of traditional ink staining procedure (TISP) is low. A modified ink staining procedure (MISP) was created to accumulate pathogen by using cell slide centrifuge. Items of RBC, WBC, and cryptococcus were observed. There were 79.98 ± 54.94 RBC and 126.98 ± 36.39 WBC in one MISP microscopic field whereas there were only 3.35 ± 2.41 RBC and 6.15 ± 1.85 WBC in one TISP microscopic field in the same sample (*200). There was statistical difference between those two methods (p = 0.000). There were 40.78 ± 13.23 mL cryptococcus in CSF processed by MISP whereas there were only 2.10 ± 1.10 mL cryptococcus in the same CSF processed by TISP. There was statistical difference between those two methods. The modified ink staining procedure contributes to the separation of cells and pathogens (such as cryptococcus).