Abstract
A fluorometric adenine assay, using glyoxal trimer to form an adenine fluorophore, was modified to allow measurement of adenine in samples of plasma and urine. The assay was set up to be accurate and precise in the 10–60 μg/ml range, but can be used at 10-fold lower concentrations. Recoveries of adenine added to plasma were above 98%, and similar additions of adenine to urine gave 93–95% recovery in the 25–100 μg/ml range. The technique may be of value in monitoring the plasma and urine of patients receiving adenine as part of a blood transfusion, or as a therapeutic treatment for metabolic diseases involving purine nucleotides.
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