Abstract

Tissue blocks impregnated with the Golgi-Kopsch method were embedded in a modified Epon formulation (1.5 parts DDSA, 1 part Epon 812, 1.9% DMP-30). Sections 150 μm thick were cut with a steel knife on a sliding microtome. The surface of the sections were counterstained with basic dyes to localize Golgi-impregnated neurons within cyto-architectonically defined areas. Neither the embedding nor counterstaining procedure appeared to adversely affect the quality of the Golgi preparation.

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