A MODIFIED APPROACH TO THE ADOPTIVE IMMUNOTHERAPY OF WALKER-256 CARCINOSARCOMA WITH A COMBINATION OF ACTIVATED MACROPHAGES AND PHOTODYNAMIC THERAPY

Abstract

Experiments were performed on five batches of Wistar inbred rats with Walker-256 carcinoma receiving sole (PDT, MAK) or therapy (PDT+MAK−A; PDT+MAK−B); the control batch (HBSS) consisted of animals with untreated tumors. The results were as follows: (a) the sole treatment (PDT, MAK) gave survival rates between 37.7 and 47.5 %, the combined therapy in five doses increased significantly (70.8 %) the survival rate of tumor bearing rats as well as the rate of complete regression (82.1%). The cell-mediated immunity test and histopathological and as well as the electron microscopy observation were in full agreement with the results above. Summing up, this results demonstrate that combined photodynamic therapy with intra and peritumoral MAK infusion stimulated cell - mediated antitumoral activity, increased survival rates and reduces incidence of Walker-256 carcinoma in rat model.