Abstract
A translational initiation sequence, conserved among soybean lipoxygenase genes, was fused with the β-glucuronidase (GUS) gene, placed under 35S cauliflower mosaic virus promoter control, then electroporated into suspension-cultured protoplasts of tobacco and rice. These constructs produced 11- and 7-fold increases in GUS activity, respectively, compared with the original GUS construct. The transcriptional activity of the modified GUS gene fused with the TATA box from a soybean lipoxygenase gene was also detected with great sensitivity. The fusion construct is useful for detectingcis-regulatory element effects on transcription, in particular, silencers.
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