A modification of the Roe procedure for determination of fructose in tissues with increased specificity

Abstract

A new modification of the procedure of Roe for determination of fructose concentrations in tissues is described. The modification shows no reaction with glucose or glucose phosphates. Tissues are homogenized in dilute perchloric acid, filtered, and reacted with alcoholic resorcinol and 30% HCl for 1 hr at 80°C. As little as 0.33 μg/ml of fructose may be reliably determined. Fructose phosphates react in this test but to a lesser extent than pure fructose.