A Model Prediction for Chenodeoxycholate Aggregate Formation

Abstract

A relationship between the chenodeoxycholate (CDC) monomer concentration and the total concentration of CDC was established using a kinetic dialysis technique. Meanwhile, the sizes of the formed simple CDC micelles were measured by a quasielastic light-scattering (QLS) technique to be nearly constant. The QLS results led to a suggestion for equilibrium models of CDC aggregate formation. According to the established relationship and the suggested models, the best curve-fitting model was selected by a least-squares technique. Furthermore, the model parameters were quantified. Based on the quantified parameters, at a minimum detectable concentration of simple CDC micelles to be ∼0.2 mM, an appropriate model corresponding concentration of CDC monomers was estimated to be ∼3.08 mM. This value is consistent with a minimum monomer CDC concentration of ∼3.13 mM for simple CDC micelle formation estimated according to the present QLS detection and the model prediction. The consistency confirms the model prediction that at a low CDC monomer concentration (<3 mM), the concentration of stable CDC dimers is much higher than that of simple CDC micelles but the contribution of simple CDC micelles to the total CDC concentration cannot be negligible.