Abstract
We have developed a modified canine model of femoral arterial thrombolysis that offers several practical advantages, including the lack of requirement for ateriography and the direct, continuous measurement of blood flow by Doppler flowmetry. In closed-chest, anesthetised dogs, a hollow catheter is advanced to either femoral artery via the left carotid artery. A flexible guidewire is advanced to the femoral artery through the catheter. The latter is withdrawn and a copper coil slipped over the guidewire, inserted through the carotid arterial incision, and pushed with the same hollow catheter to the femoral artery. The guidewire is withdrawn and replaced by a small catheter if local instillation of a thrombolytic agent is desired. The hollow, guiding catheter is also withdrawn, restoring flow through the artery. An occlusive platelet-rich thrombus forms within 12 ± 1 min. Both intravenous and intraarterial streptokinase or tissue plasminogen activator lyse these thrombi dose-dependently. The direct, continuous measurement of femoral arterial blood flow enables the exact moment of thrombolysis and reocclusion as well as the magnitude of blood flow after lysis to be quantified accurately. Cyclic, repetitive reocclusions, indicative of thrombus formation and dislodgement, often precede permanent reocclusion, making the model suitable for evaluation of potential antithrombotic agents.
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