Abstract
Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging human pathogen, endemic in areas of China, Japan, and the Korea (KOR). It is primarily transmitted through infected ticks and can cause a severe hemorrhagic fever disease with case fatality rates as high as 30%. Despite its high virulence and increasing prevalence, molecular and functional studies in situ are scarce due to the limited availability of high-titer SFTSV exposure stocks. During the course of field virologic surveillance in 2017, we detected SFTSV in ticks and in a symptomatic soldier in a KOR Army training area. SFTSV was isolated from the ticks producing a high-titer viral exposure stock. Through the use of advanced genomic tools, we present here a complete, in-depth characterization of this viral stock, including a comparison with both the virus in its arthropod source and in the human case, and an in vivo study of its pathogenicity. Thanks to this detailed characterization, this SFTSV viral exposure stock constitutes a quality biological tool for the study of this viral agent and for the development of medical countermeasures, fulfilling the requirements of the main regulatory agencies.
Highlights
Severe fever with thrombocytopenia syndrome virus (SFTSV; Bunyavirales: Phenuiviridae: Dabie bandavirus [1]) is an emerging tick-borne virus that is distributed throughout much of Eastern Asia and causes severe fever with thrombocytopenia syndrome (SFTS: ICD-11: 1D4E; ICD-10: A93.8) [2].Clinical signs include acute fever, thrombocytopenia, leukocytopenia, and gastrointestinal symptoms; severe presentations can progress to organ failure and death (12–30% case fatality rate) [3]
Day 6 after exposure, medium was removed, and cells were washed with phosphate-buffered saline (PBS, Thermo Fisher Scientific) before the entire dish and lid were submerged in Transmission electron microscopy (TEM) fixative
Viral genomes from the serum samples of the patient, from the RT-qPCR-positive tick pool, and from the SFTSV isolate from Passage 3 were obtained through target-enrichment high-throughput sequencing (HTS)
Summary
Unai Perez-Sautu 1,† , Se Hun Gu 2,† , Katie Caviness 1 , Dong Hyun Song 2 , Yu-Jin Kim 3 , Nicholas Di Paola 1 , Daesang Lee 2 , Terry A. Chitty 1 , Elyse Nagle 1 , Heung-Chul Kim 4 , Sung-Tae Chong 4 , Brett Beitzel 1 , Daniel S. Reyes 1 , Courtney Finch 5 , Russ Byrum 5 , Kurt Cooper 5 , Janie Liang 5 , Jens H. Kuhn 5 , Xiankun Zeng 6 , Kathleen A. Integrated Research Facility at Fort Detrick (IRF-Frederick), Division of Clinical Research (DCR), National.
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