A model for determination of total body protein synthesis based upon compartmental analysis of the plasma [ 15N] glycine decay curve

Abstract

A model for whole body glycine nitrogen flux based on the compartmental analysis of plasma [ 15N] glycine decay curves is described and used for the measurements of (1) total body glycine nitrogen flux and the components of this flux in three healthy young adults and (2) total body protein synthesis based on the conversion of 15N to excretory products, ie, the sum of urinary [ 15N] urea and 15NH 3 and the amount of labeled urea remaining in the body at five hours following administration of [ 15N] glycine. The mean glycine nitrogen flux was 3.93 ± 0.42 mg N · kg −1 · h −1 (SEM). The major components of this flux are de novo synthesis of glycine, which accounts for 18% to 27%, and release from protein breakdown, which accounts for 62% to 73%. The outward pathways of glycine from the total body free glycine pool are conversion to other amino acids and oxidation to excretory end products (30% to 42%) and incorporation into protein, which accounts for 45% to 61% of glycine N loss from the metabolic pool. The mean rate of total body protein synthesis as determined by compartmental analysis was 3.56 g protein · kg −1 · day −1. The results that were obtained for protein synthesis and whole body glycine kinetics accord well with previous studies in normal adults, using the stochastic model.