Abstract
A model for detection of early replication bands in cultured fish
Highlights
Since the first attempts to obtain banding patterns in human chromosomes by means of differential staining after incorporation of bromodeoxyuridine (BrdU) (Latt 1973; Dutrillaux and Lejeune, 1973), other authors have employed this technique to obtain R- and G-bands in mammalian chromosomes.At present, the BrdU techniques are commonly used because of their high sensitivity in the detection of DNA replication and because of their more resolutive banding patterns.Obtaining such patterns in fish chromosomes using BrdU methodology has not been tried very often
The present paper describes a model for detection of R-bands in Rutilus alburnoides (Teleosteens, Cyprinidae), using BrdU methodology, and the modified techniques of Delany and Bloom (1984) and Giles et al (1988)
The cell cycle duration was calculated according to Klingerman et al (1984), using the following experimental conditions: a dose of 0.8 mg of BrdU per gram of fish and a temperature of 25-27°C
Summary
The BrdU techniques are commonly used because of their high sensitivity in the detection of DNA replication and because of their more resolutive banding patterns. Obtaining such patterns in fish chromosomes using BrdU methodology has not been tried very often. Delany and Bloom (1984) established R-banding patterns in Salmo gairdneri, and Giles et al (1988) did the same in Scorpaenids. The present paper describes a model for detection of R-bands in Rutilus alburnoides (Teleosteens, Cyprinidae), using BrdU methodology, and the modified techniques of Delany and Bloom (1984) and Giles et al (1988) Using trypsin and BrdU incorporation, Lingyun (1986) obtained the G-banding pattern in Monopterus albus. Delany and Bloom (1984) established R-banding patterns in Salmo gairdneri, and Giles et al (1988) did the same in Scorpaenids.
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