Abstract
F I aj s han sM.: A Model Approach to Distinguish Diploid and Triploid Fish by Means of Computer-assisted Image Analysis. Acta vet. Brno 1997,66: 101-110. A model approach to distinguish diploid and triploid fish rapidly upon computer-assisted image analysis of erythrocyte nuclear dimensions in blood smear samples was exemplified on measurements of 13 diploid and 13 artificially-induced triploid wels (Silurus glanis). Analysis of variance test confirmed that nuclear area, perimeter, nuclear major axis and average nuclear size were the decisive dimensions for such approach (P < 0.000 I). The respecti ve values (mean ± SD) for diploids and triploids were registered as follows: nuclear area 11.09 ± 1.26 11m2 and 17.34 ±2.09 11m2; nuclear perimeter 12.67 ± 0.80 11m and 16.24 ± 1.07 11m; nuclear major axis 4.62 ± 0.35 11m and 6.17 ± 0.47 11m and average nuclear size 4.0 I ± 0.22 11m and 5.12 ± 0.31 11m. This approach was found a rapid and precise alternative to other currently used methods of ploidy level identification in fish. Triploidy, image analysis, erythrocytes, nuclear dimensions, Silurus glanis
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