Abstract
Although ultrasound cavitation must be avoided for safe diagnostic applications, the ability of ultrasound to disrupt cell membranes has taken on increasing significance as a method to facilitate drug and gene delivery. A new ultrasonic resonance driving method is introduced to penetrate rigid wall plant cells or oocytes with springy cell membranes. When a reasonable design is created, ultrasound can gather energy and increase the amplitude factor. Ultrasonic penetration enables exogenous materials to enter cells without damaging them by utilizing instant acceleration. This paper seeks to develop a miniature ultrasonic probe experiment system for cell penetration. A miniature ultrasonic probe is designed and optimized using the Precise Four Terminal Network Method and Finite Element Method (FEM) and an ultrasonic generator to drive the probe is designed. The system was able to successfully puncture a single fish cell.
Highlights
Single cell membranes are selective in that only water, ions and small molecules are able to pass through while the exchange of other materials is hindered
Since many important biochemical reactions happen inside the cell, numerous techniques have been developed for the transmission of exogenous materials through cell membranes
The acceleration of the probe must exceed the threshold value of 50,000 g in order to ensure the penetration of a cell, so the appropriate frequency and amplitude were calculated in the paper
Summary
Single cell membranes are selective in that only water, ions and small molecules are able to pass through while the exchange of other materials is hindered. Since many important biochemical reactions happen inside the cell, numerous techniques have been developed for the transmission of exogenous materials through cell membranes. These techniques include electroporation [1,2], liposome transfection [3], microinjection [4] and so on. In 2005, Obataya [5] was able to successfully penetrate a cell membrane in atomic force microscopy (AFM) via a Si needle. The patch clamp technique [7], which won the inventor the Nobel Prize in 1991, further simplified single cell penetration and organelle analysis
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