Abstract
In this manuscript, we establish the susceptibility of the N-methyldiaminobenzoyl linker to undergo undesired acylation during standard peptide capping with acetic anhydride. Successive capping treatments led to problematic levels of linker incapacitation. We describe a mild, inexpensive alternative capping strategy that is completely selective for the N terminus with no acylation of the linker detected for any of the substrates evaluated. The utility of this protocol is demonstrated via the synthesis of the CAPA-PVK-1 consensus sequence of the C. elegans neuropeptide-like protein precursor peptide NLP-44.
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