Abstract
A fluorimetric assay was developed to measure halide release from halogenated compounds being degraded by microbes. The method relies on the property of halides to quench the fluorescence of 6-methoxy- N-(3-sulfopropyl)quinolinium (SPQ) by collision quenching. The assay shows a wide response to halide concentration (1–500 mM) and tolerates a wide pH range. Furthermore, it is simple to use, has the potential for automation and uses an inexpensive non-toxic reagent.
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