Abstract
Radial diffusion of enzymes into a casein-containing gel is a fast and inexpensive technique for determination of proteolytic enzymes. A simple method for the preparation of substrate plates with a homogeneous distribution of caseinate and buffered at selected pH values between 2.8 and 4.8 has been described in this communication. By determination of pepsin in caseinate gels the accuracy was better than that described for photometric pepsin assays, and the sensitivity was 300 times greater.
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