Abstract
Abstract A large number of processes underlying immune system biology are dependent on leukocyte adhesion and neutrophil rolling phenomena. In vitro, the control and quantification of the shear stress applied during such experiments is performed using laminar flow chambers. Despite their wide use, flow chambers suffer from low throughput and significant sterilization and setup times. Here we report on the development of a microfluidic platform that offers significant advantages over existing systems. Microfluidic designs enable the simultaneous assaying of a number of different shear regimes, while reducing reagent consumption. We present experimental data for cell adhesion and rolling neutrophil assays comparing traditional flow chamber experiments to microfluidic experiments at multiple shear force points, including time-lapse images. Biomimetic flow patterns and a wide range of shear protocols are also presented.
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