A Microfluidic Paper-Based Analytical Device for Type-II Pyrethroid Targets in an Environmental Water Sample.

Sumate Pengpumkiat,Pornpimol Kongtip,Jintana Nammoonnoy,Watcharaporn Wongsakoonkan,Pajaree Konthonbut

doi:10.3390/s20154107

Sumate Pengpumkiat, Pornpimol Kongtip + Show 3 more

Open Access

https://doi.org/10.3390/s20154107

Copy DOI

Abstract

A detection method for type-II pyrethroids in an environmental water sample using a microfluidic paper-based analytical device (µPAD) is reported here. The detection approach is based on the formation of cyanide from the hydrolysis of type-II pyrethroids and the colorimetric detection of cyanide on a layer-based µPAD. Parafilm and inexpensive laminating pouches were used to create a hydrophobic barrier for the assay on the µPAD. This detection approach was selective to type-II pyrethroids in water for which an environmental water sample was tested. The calibration curves for cypermethrin, deltamethrin, cyhalothrin, and fenvalerate ranged from 2 to 40 µg/mL without sample preconcentration. The lower concentrations of type-II pyrethroids can be assessed by including a preconcentration step prior to the detection on a µPAD. This detection system provides an alternative platform for fast, semiquantitative testing for pesticide contamination in environmental surface water by allowing for portability, low reagent/sample consumption, and low-cost testing.

Highlights

A microfluidic paper-based analytical device was initially introduced as a point-of-care testing (POCT) device by the Whitesides research group in 2007 [1]
The detection principle of this work was based on the hydrolysis of type-II pyrethroid pesticides the μPAD
We demonstrated the performance of type-II pyrethroid detection on a μPAD without sample the performance detection on a μPAD

Summary

Introduction

A microfluidic paper-based analytical device (μPAD) was initially introduced as a point-of-care testing (POCT) device by the Whitesides research group in 2007 [1]. It integrates sample preparation, chemical reaction, and detection in a single step. In terms of pesticide analysis, organophosphate and carbamate can be analyzed on a μPAD using chemicals and nanoparticles [8,9,10], targeting the inhibition of acetylcholinesterase (AChE) activity by the pesticides for the detection principle [11,12,13,14,15,16,17,18,19]

Methods

Results

Conclusion