Abstract
Antioxidants are an important substance that can fight the deterioration of free radicals and can easily oxidize when exposed to light. There are many methods to measure the antioxidant activity in a biological sample, for example 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity test, which is one of the simplest methods used. Despite its simplicity, the organic solvent that has been used to dilute DPPH is easily evaporated and degraded with respect to light exposure and time. Thus, it needs to be used at the earliest convenient time prior to the experiment. To overcome this issue, a rapid and close system for antioxidant activity is required. In this paper, we introduced the Lab-on-a-Disc (LoD) method that integrates the DPPH antioxidant activity test on a microfluidic compact disc (CD). We used ascorbic acid, quercetin, Areca catechu, Polygonum minus, and Syzygium polyanthum plant extracts to compare the results of our proposed LoD method with the conventional method. Contrasted to the arduous laborious conventional method, our proposed method offer rapid analysis and simple determination of antioxidant. This proposed LoD method for antioxidant activity in plants would be a platform for the further development of antioxidant assay.
Highlights
Free radicals are atoms with unpaired valence electrons that cause its chemical instability and reactivity
When free radical production becomes excessive in the body, it may cause oxidative stress, a condition whereby the body cannot counter-attack the production of free radicals, leading to cellular damage and cell death [1]
The DPPH solutions need to be diluted in organic solvent and prepared fresh before running the antioxidant activity test
Summary
Free radicals are atoms with unpaired valence electrons that cause its chemical instability and reactivity. The DPPH solutions need to be diluted in organic solvent and prepared fresh before running the antioxidant activity test. LoD decreases the amount of reagent, sample usage and total processing time during the experiments It offers parallel or sequential loading of solutions. LoD skips multiple sample preparations and pipetting steps, which reduces human-prone handling error. The design consist of a two microfluidic compartment; the first part contain human liver enzyme that mimic liver metabolism and the second part is DPPH detection of antioxidant activity of food components. In his design, the liver enzyme fractions were immobilized and the reaction with the DPPH solution were tested. Our proposed LoD method has been tested on ascorbic acid, quercetin, Areca catechu, Polygonus minus, and Syzygium polyanthum plant extract
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