Abstract
The application of microfluidic technology to manipulate cells or biological particles is becoming one of the rapidly growing areas, and various microarray trapping devices have recently been designed for high throughput single-cell analysis and manipulation. In this paper, we design a double-slit microfluidic chip for hydrodynamic cell trapping at the single-cell level, which maintains a high capture ability. The geometric effects on flow behaviour are investigated in detail for optimizing chip architecture, including the flow velocity, the fluid pressure, and the equivalent stress of cells. Based on the geometrical parameters optimized, the double-slit chip enhances the capture of HeLa cells and the drug experiment verifies the feasibility of the drug delivery.
Highlights
Microfluidic technology has become a research hotspot in recent years [1], and has received significant attention in various fields, including biomedical science [2] and biochemical studies [3]
Compared with conventional methods [6], microfluidics can meet this request of high-throughput single cell analysis
Microfluidic chip technology in single cell analysis is widely used for intercellular dynamics research, drug screening, and immune response, which greatly promotes the progress of biomedical and cellular biology
Summary
Microfluidic technology has become a research hotspot in recent years [1], and has received significant attention in various fields, including biomedical science [2] and biochemical studies [3]. Among these fields, cell biology is an important field of microfluidic technology application [4]. Compared with conventional methods [6], microfluidics can meet this request of high-throughput single cell analysis. It has various advantages, such as reagent volume control, cell handling, device automation, and multiple component integration [7]. Microfluidic chip technology in single cell analysis is widely used for intercellular dynamics research, drug screening, and immune response, which greatly promotes the progress of biomedical and cellular biology
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