Abstract
This paper developed a microfluidic chip for whole genome sequencing (WGS) of circulating tumor cells (CTC) at single-cell level. For the first time, all processing steps between collecting blood sample and feeding prepared sample to sequencer are finished in a single microfluidic chips. The microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. The microfluidic chip was validated by clinical samples. Our results demonstrate that for the first time, the aim of “whole blood in, WGA product out” has been realized at single CTC level with clinical samples, in a microfluidic chip.
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